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LGC
主营:主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
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当前位置: 首页 > 产品中心 > Cloning_kit > Lucigen/Fast Link™DNA连接试剂盒/LK6201H/100连接
商品详细Lucigen/Fast Link™DNA连接试剂盒/LK6201H/100连接
Lucigen/Fast Link™DNA连接试剂盒/LK6201H/100连接
Lucigen/Fast Link™DNA连接试剂盒/LK6201H/100连接
商品编号: LK6201H
品牌: LGC
市场价: ¥5000.00
美元价: 3000.00
产地: 美国(厂家直采)
公司:
产品分类: 克隆试剂盒
公司分类: Cloning_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
  • ClonewithspeedandefficiencyFormerly from Epicentre
  • TransformintoE.coliwithoutclean-upordesalting

Applications

  • Blunt-endandTAcloningofPCRproducts.
  • Ligationofnext-gensequencingadaptorstoblunt-endedDNA.
  • GenomicDNAcloningandsubcloning.
  • BAC/fosmidlibraryconstruction.
  • CDNAcloning.
  • Linkerligation.
TheFast-Link™DNALigationKitusesahigh-qualityligase(Fast-LinkT4DNALigase),thatwasclonedatEpicentreandformulatedtoprovideextremelyrapid,high-efficiencyDNAligation.Cohesive-endligationscanbeperformedin5minutesatroomtemperature.Incontrasttootherligases,itisnotnecessarytodesaltFast-Linkligationreactionspriortotransformationofelectrocompetentorchemicallycompetentcells.TheFast-LinkKitcanbeusedforroutineandhigh-throughputDNAcloning.
TypeofEndLigationTime%WhiteColoniesRecombinantsperµgDNA
Overhang5min.932.0x106
Blunt15min.714.4x105
PCRproductwith3´-Aoverhang1hr.681.2x104

Table1.Fast-Link™representativeresults.

Benefits

  • Cohesive-endligationsin5minutesatroomtemperature(Fig.1).
  • Blunt-endligationsin15minutesatroomtemperature(Fig.2).
  • LigationofPCRproductwithA-overhangsin1hourat16°C.
  • Highligationefficiency(Table1).
  • Desaltingofligationproductsisnotneededpriortotransformationofelectrocompetentorchemicallycompetentcells.
  • BACligationwithinserts>100kbcanbeperformedin4hours,insteadofovernightreactionsaswithotherligases.
Figure1.TimecourseforcohesiveendligationusingtheFast-Link™Kit.LamBDaHindIIIMarkerswereligatedusing2unitsofFast-LinkDNALigase(Lanes2-6).LaneM,Kilobaseladder;lane1,noenzyme.Figure2.Timecourseforblunt-endligationusingtheFast-Link™Kit.pUC19DNAdigestedwithPvuIIwasligatedusing2unitsofFast-LinkDNALigase(lanes1-6).LanesM,Kilobaseladder.

ORDERINFORMATION

Contents:Fast-Link™DNALigase,Fast-Link™10XLigationBuffer,10mMATP.
品牌介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。