Lucigen/T4多核苷酸激酶/30061-3/5000 U
市场价：
¥7360.00
美元价：
4416.00
产品分类：
DNA修饰酶
公司分类：
DNA_modification_enzyme
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- RecombinantT4PolynucleotideKinase(T4PNK).
- Applicationsincludeend-labelingandinsertpreparationforcloning.
T4PolynucleotideKinasecatalyzesthetransferandexchangeofgamma-phosphateofATPtothe5´-hydroxylterminusofpolynucleotides(double-andsingle-strandedDNAandRNA)andnucleoside3´-monophosphates.
Source:
PurifiedfromastrainofE.colithatcarriesaT4PolynucleotideKinaseexpressionplasmid.
Applications:
- End-labelingDNAforprobesandDNAsequencing(1)
- Additionof5´-phosphatestooligonucleotidestoallowsubsequentligation(2)
Heatinactivation:70°Cfor15minutes.
Storage:
Enzymeandbuffershouldbestoredat-20°C.Enzymeisstablefor12monthsifhandledproperly.
AdditionalInformation:
- AmmoniumionsarepotentinhibitorsofT4PolynucleotideKinase. DNAshouldnotbeprecipitatedfromordissolvedinbufferscontainingammoniumsalts.
- Protruding5’-overhangsaremoreefficientlyphosphorylatedthanblunt-orrecessed5’overhangs.
References:
- Sambrook,J.andRussell,W.(2001)MolecularCloning:ALaboratoryManual,(3rded.),9.55-9.56,9.68-9.69.
- Sambrook,J.andRussell,W.(2001)MolecularCloning:ALaboratoryManual,(3rded.),13.16-13.17.
品牌介绍
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
