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LGC
主营:主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
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当前位置: 首页 > 产品中心 > DNA_extraction > 卢西根/MasterPure™ 革兰氏阳性DNA纯化试剂盒/MGP04100/100纯化
商品详细卢西根/MasterPure™ 革兰氏阳性DNA纯化试剂盒/MGP04100/100纯化
卢西根/MasterPure™ 革兰氏阳性DNA纯化试剂盒/MGP04100/100纯化
卢西根/MasterPure™ 革兰氏阳性DNA纯化试剂盒/MGP04100/100纯化
商品编号: MGP04100
品牌: LGC
市场价: ¥5620.00
美元价: 3372.00
产地: 美国(厂家直采)
公司:
产品分类: DNA提取纯化试剂
公司分类: DNA_extraction
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PurifygenomicDNAfromchallengingGrampositivebacteriaforawidevarietyofmolecularBIOLOGyapplications

  • Epicentre ProductProducesclean,highmolecularweightgenomicDNAreadyforPCRandothermolecularbiologyapplicationssuchasrestrictiondigestion,cloning,andSouthernblotting
  • Scaleablemethodforlargesamplevolumeswithnowastingofreagentswithsmallersamples
  • Completekitincludeslysozyme;noseparatepurchaserequired
  • Robustkitworksonavarietyoftestedspecies
  • FlexIBLekitalsopurifiesgDNAfromGramnegativebacteria

TheMasterPure™GramPositiveDNAPurificationKitprovidesallofthereagentsneededtopurifyDNAfromGrampositivebacteria.ThesebacterialysemorereADIlyaftertreatmentwithReady-Lyse™LysozymeandtheGram-PositiveCellLysisSolutionincludedinthekit.Ready-LyseLysozymeisastablesolutionofarecombinantlysozymefromanonmammalian,nonaviansource.IthashighspecificactivityanddoesnotbindDNA.1,2

ExamplesofGram-positivebacteriatestedwiththiskitarelistedinTable1.ThesizerangeofDNApurifiedbytheMasterPureGramPositiveDNAPurificationKitisshowninFig.1.TheDNApurifiedusingthekitissuitableforPCRanalysis(Fig.2),andothermolecularbiologyapplications.

SpeciesCultureMediumReady-Lyse™IncubationDNAYieldµg/ml
BacillussubtilisBrain-HeartInfusion(BHI)30min9.0
Listeria monocytogenesBHIOvernight3.3
StaphylococcusaureusBHINotneeded8.0
StreptococcusmutansTodd-HewittOvernight3.0
LactococcuslactisM1730min1.1

Table1.IncubationtimesneededforDNArecoveryfrom1mlofGrampositivebacterialculture.


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Figure1.ElectrophoreticanalysisofDNApurifiedusingtheMasterPure™GramPositiveDNAPurificationKit.PanelA:DNApurifiedfromB.subtilis(ATCC6051)wasseparatedona1%agarosegelandstainedwithSYBR®Gold.LaneM,kilobaseladder;lane1,300ngofB.subtilisDNA.PanelB:Pulse-fieldgelelectrophoresisofB.subtilisDNA.LaneM,PhagelamBDaladder;lane1,300ngofDNA;lane2,600ngofDNAFigure2.PCRanalysisofDNApurifiedusingtheMasterPure™GramPositiveDNAPurificationKit.B.subtilisDNAwasamplifiedbyPCRusingBacF/BacRprimers,andtheproductswereanalyzedbyagarosegelelectrophoresis.LaneM,100-bpladder;lane1,PCRproductfrom0.5ngofB.subtilisDNA;lane2,no-templatecontrol.

References

  1. Hoffman, L. and Jarvis, B. (2003) Epicentre Forum 10(3), 3.
  2. Jarvis,B.andHoffman,L.(2004)EpicentreForum11(3),7.
  3. Luna,V.A.etal.(2006)J.Clin.Microbiol.44,2367.
  4. Li,J.etal.(2007)Immunol.75,1811.

ORDERINFORMATION

Contents:Gram-PositiveCellLysisSolution,MPCProteinPrecipitationReagent,Ready-Lyse™Lysozyme,ProteinaseK,TEBuffer,RNaseA.
品牌介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。