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商品详细Lucigen/Bst DNA聚合酶，核酸外切酶-8000 U/ml/30027-2/10000（5 x 2000单位）

Lucigen/Bst DNA聚合酶，核酸外切酶-8000 U/ml/30027-2/10000（5 x 2000单位）

商品编号： 30027-2

品牌： LGC

市场价： ¥6340.00

美元价： 3804.00

产地：美国(厂家直采)

公司：

产品分类：恒温扩增

公司分类： RPA

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Highstranddisplacementactivity.
UsedinisothermalamplificationandNextGenerationSequencing.

FreeSampleAvailable

HighspecificactivityforDNAamplificationandsequencing.

Applications

Stranddisplacementamplification*
DNAsequencingthroughhighGCregions(1,2)
RapidsequencingfromnanogramamountsofDNAtemplate(3)

BstDNAPolymerase,ExonucleaseMinus,isarecombinantformofthe67kDaBacillusstearoThermophilusDNAPolymeraseprotein(largefragment).Theenzymehas5’-3’polymeraseactivityandstranddisplacementactivity,butitlacks3’-5’exonucleaseactivity.Italsohasreversetranscriptionactivity.

Lucigen'sBstDNAPolymerase,ExonucleaseMinus,hashigherstranddisplacementactivitythanthatofothersuppliers(Figure1).Theenzymecanbeusedinnucleicacidamplificationmethods*suchasisothermalamplification,wholegenomeamplification(WGA),andmultipledisplacementamplification(MDA).ItalsocanbeusedinNextGenerationsequencing.

Thisenzymehasoptimalactivityat65°C.ItissuitableforsequencingDNAwithhighGCcontentandsecondarystructures.Itisavailableinconcentrationsof8,000U/mlor50,000U/ml.

BstDNAPolymerase,ExonucleaseMinus,issuppliedwith10XDNAPolymeraseBufferB,composedof200mMTris-HClpH8.8,100mM(NH₄)₂SO₄,100mMKCl,20mMMgSO₄,and1.0%TritonX-100.

Lucigen Bst versus competitors.

Figure1.LucigenBstDNAPolymerase,ExonucleaseMinus,possessesgreaterstrand-displacingpolymeraseactivity.M13singlestrandedDNAwasincubatedwithorwithout8unitsofBstDNAPolymerase(+/-Bst)inreactionbuffersuppliedbythemanufacturer,withorwithoutreplicationprimer(+/-primer)for30minutesat65°C.MW,1kbladder.

Tipsforuse:

Requires0.1%TritonX-100forlongtermstorage.
Reactiontemperaturesabove70°Carenotrecommended.
BstDNAPolymerasecannotbeusedforthermalcyclesequencing.

HeatInactivation:80ºCfor20min.

References:

Griffin,H.andGriffin,A.(1994)PCRTechnology,228-229.
McClary,J.etal.(1991)J.DNASequencingandMapping,1,173-180.
Mead,D.A.etal.(1991)Biotechniques,11,76-87.

*Note:
Someusesforthisproductmayrequirelicenses.Lucigendoesnotencourageorsupporttheunauthorizedorunlicenseduseofpatentednucleicacidamplificationprocessesforisothermalamplification,wholegenomeamplification(WGA),multipledisplacementamplification(MDA),andNextGenerationsequencing.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.Ifthepurchaserisnotwillingtoaccepttheseuselimitations,LucigenCorporationiswillingtoacceptreturnoftheproductforafullrefund.

ORDERINFORMATION

BstDNAPolymerase,ExonucleaseMinusisavailableintwoconcentrations,8,000U/mland50,000U/ml,inastoragebufferof10mM Tris-HCl,pH7.5,50 mM KCl,1 mM DTT,0.1 mM EDTA,0.1% TritonX-100,and50% Glycerol.Alsosuppliedis10XDNAPolymeraseBufferB,composedof200 mM Tris-HClpH8.8,100 mM (NH₄)₂SO₄,100 mM KCl,20 mM MgSO₄, and1.0 % TritonX-100.

品牌介绍

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。

公司介绍

品牌分类

灯泡和等温放大天然气产品缓冲液和试剂哺乳动物的表达 DNA核酸酶高效体外转录试剂盒大肠杆菌聚合酶 RNA扩增与标记

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.lucigen.com