Lucigen/MaxPlax&trade；Lambda包装提取物/MP5105/5提取物
市场价：
¥3860.00
美元价：
2316.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
MaximizedpackagingefficiencyoflamBDa,cosmid,orfosmidDNAfortheconstructionoflargeinsertgenomiclibraries.
ConstructgenomiclibrariesfrommethylatedorunmethylatedDNAwithequalefficiencyduetothelackofallknownrestrictionactivitiesintheextracts.- Packagingefficienciesofupto3×109pfu/µgDNA.
- Simplifiedprotocoldoesnotrequirepremixingofdifferentextractcomponentsbeforeuse.
MaxPlax™LambdaPackagingExtractsoffermaximuminvitropackagingefficienciesofcossite-containingmethylatedorunmethylatedDNA.TrADItionallambdapackagingextractsarederivedfromtwocomplementarylysogenicE.colistrains,BHB2690andBHB2688,asdescribedbyHohn.1MaxPlaxExtractsusearestriction-freeE.coliK-12strain,NM759,inplaceofstrainBHB2690.2ThesonicatedextractsofNM759arecombinedwithextractsofBHB2688toproduceMaxPlaxExtracts.
EachlotofMaxPlaxExtractsistestedandguaranteedtomaintainapackagingefficiencyofgreaterthan1×109pfu/µgofControlLigatedLambdaDNAfor1yearfromthedateofpurchaseifstoredproperly.ACertificateofAnalysisstatingactualpackagingefficiency(pfu/µgDNA)isprovidedwitheachlotofMaxPlaxExtracts.
| Table1.PackagingofmethylatedandunmethylatedDNAusingMaxPlax™LambdaPackagingExtracts. | |
| CosmidInsert | Pfu/µgDNA |
| UnmethylatedDNA | 3.5x107 |
| MethylatedDNA | 3.9x107 |
References
- Hohn,E.G.(1979)MethodsEnzymol.68,299.
- Gunther,E.G.etal.(1993)NucleicAcidsRes.21,3903.
- Fiandt,M.(2000)EpicentreForum7(3),14.
ORDERINFORMATION
Contents:PredispensedMaxPlax™Extracts,ControlLambdaDNA,ControlHostCells(E.coli).品牌介绍
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
