ImprovedFosmidCloning
Lucigen’sCopyRight®v2.0FosmidCloningKitwiththepSMART®FOSvectorandReplicator™FOSstraincontainsthemostadvancedfosmidcloningdeveloped.TheCopyRightv2.0FosmidKityieldsmanymorerecombinantsfromlimitedstartingDNA(Figure1). ThevectorincorporatesCopyRighttranscriptionterminatorstostabilizeinserts,whichresultsincompletefosmidlibrarieswithoutmissingclones.False-negativesandfalse-positivesaredramaticallyreduced,andempty-vectorbackgroundisessentiallyeliminated(Figure2).ControllablegeneticelementsinthepSMARTFOSvector(Figure3)andReplicatorFOScellsallowamplificationofvectorcopynumberforhighplasmidyields(Figure4).
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Figure1.HigherrecombinantyieldswithalimitingamountofDNA. Lucigen’sCopyRightFosmidkitcomparedtoEpicentre’sCopyControlkit. |
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Figure2.ZerobackgroundwiththepSMARTFOSvector(left)comparedtotheCopyControl™pCC1FOS™vector(right)(Epicentre). |
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Figure3.CopyRightv2.0pSMARTFOSvector.sacB,sucrasegene;lacZ,lacZalphapeptidegene;Cm^r-chloramphenicolresistancegene;cosN-lamBDapackagingsignal;parA,B,C-partitiongenes;ori2,repE[notshownindrawing],IncC-single-copyoriginofreplication;oriV-inducibleoriginofreplication.Approximatepositionsofsequencingprimersandtranscriptionterminators(T)areindicated.ThelacZ/sacBstufferregioniscompletelyremovedduringvectorprocessing. |
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Figure4.CopynumberamplificationoffosmidclonesfromtheCopyRightv2.0FosmidKit(Replicatorcells)wasmoreconsistentandrobustthantheCopyControlFosmidkit(EPI300cells). |
ConvenientSuccess
Efficientlyobtainyourcloneorcreateyourlibrary—theCopyRightv2.0CloningKitseliminatetediousvectorandcompetentcellpreparation,aswellastime-consumingQCtestingexperiments. Kitsincludeoptimizedreagents,ligation-readyvector,optimizedcells,detailedinstructions,andtrouble-shootingguide.
