
Lucigen/Fast Link™DNA连接试剂盒/LK0750H/50连接
市场价:
¥3060.00
美元价:
1836.00
产品分类:
克隆试剂盒
公司分类:
Cloning_kit
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
- Clonewithspeedandefficiency
- TransformintoE.coliwithoutclean-upordesalting
Applications
|
Table1.Fast-Link™representativeresults. |
Benefits
- Cohesive-endligationsin5minutesatroomtemperature(Fig.1).
- Blunt-endligationsin15minutesatroomtemperature(Fig.2).
- LigationofPCRproductwithA-overhangsin1hourat16°C.
- Highligationefficiency(Table1).
- Desaltingofligationproductsisnotneededpriortotransformationofelectrocompetentorchemicallycompetentcells.
- BACligationwithinserts>100kbcanbeperformedin4hours,insteadofovernightreactionsaswithotherligases.
![]() | Figure1.TimecourseforcohesiveendligationusingtheFast-Link™Kit.LamBDaHindIIIMarkerswereligatedusing2unitsofFast-LinkDNALigase(Lanes2-6).LaneM,Kilobaseladder;lane1,noenzyme. | ![]() | Figure2.Timecourseforblunt-endligationusingtheFast-Link™Kit.pUC19DNAdigestedwithPvuIIwasligatedusing2unitsofFast-LinkDNALigase(lanes1-6).LanesM,Kilobaseladder. |
ORDERINFORMATION
Contents:Fast-Link™DNALigase,Fast-Link™10XLigationBuffer,10mMATP.品牌介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。