- Directreplacementsforstandardcloningstrains(e.g.,DH5α™,DH10B™,JM109,TOP10,XL1-Blue,etc.).
- Optimizedgeneticsforhighyields:endonucleaseandrecombinationminus,forblue/whitescreening-capable.
Lucigen’sE.clonicompetentcellssharethemostusefulgeneticelementsofstandardcloningstrainslikeDH5α™anddirectlyreplacethemincloningprotocols. E.clonichemicallycompetentcellsprovidetheperformanceresearchersneedwitheaseofuse—onlycommonlaboratoryequipmentisrequired. Thesecellsprovidesolutionsforawiderangeofapplicationsateconomicalprices.
E.cloni5-alphaChemicallyCompetentCells>1×108cfu/µgDNA
Usefulforroutinecloning,subcloning,andplasmidisolationwithorwithoutblue/whitescreening.
CustomCompetentCells
BulkLucigencellsoryourstrainofE.coli,madetothehighestpossIBLetransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellspagefordetailedinformation.
Table1.E.cloniCompetentCellsforCloning
E.cloniCellLines | TransformationEfficiency | CloningMethylatedDNA | BAC,CosmidCloning | Blue/WhiteScreening |
5-alphaChemicallyCompetent | >1×108cfu/µgDNA | NO | NO | YES |
ORDERINFORMATION
CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.
PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.
