- Cellsthatenablethemostchallenginglibraryconstructionandscreening.
- Highestavailabletransformationefficienciesforlibraryconstructionandscreening.
- OptimizedforhighyieldsofDNA.
LucigenofferstwobacterialstrainsdesignedforBAClibraryconstructionortransformationoflargeconstructs:
BAC-OptimizedReplicator™v2.0ElectrocompetentCells(>1×1010cfu/µgpKanRDNA). Thesecellsarerequiredforon-demand,copynumberamplificationofthepSMART®BACorpEZ™BACvectorsintheCopyRight®Kits,therebyincreasingDNAyields. BAC-OptimizedReplicatorCellsarecomponentsofLucigen’sCopyRight®CloningKits.
E.cloni10GBAC-OptimizedElectrocompetentCells(>1×107cfu/µg150kbBACDNA;>1×1010cfu/µgpUCDNA).ThefirstcompetentcellsdevelopedbyLucigenspecificallyforlargeDNAcloning. Manufacturedusinganovelprocedurethatmaximizestransformationefficiencieswithverylargeinserts,10GBAC-OptimizedCellsareanexcellentchoiceforBAClibraryconstructionandlargeconstructtransformation.
Table1.E.cloniCompetentCellsforBACorLargeFragmentCloning
E.cloniCellLines | TransformationEfficiency | CloningMethylatedDNA | BAC,CosmidCloning | Blue/WhiteScreening |
10GBAC-OptimizedElectrocompetent | >1×1010 | YES | YES | YES |
BAC-OptimizedReplicatorv2.0Electrocompetent | >1×1010 | YES | YES | YES |
ORDERINFORMATION
CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube)orFIVERs(5transformationspertube),asindicated.RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.
