- CellsrequiredfortransformationandpropagationofpJAZZ®Vectors.
- Highestavailabletransformationefficienciesforlibraryconstructionandscreening.
- OptimizedforhighyieldsofDNA.
BigEasy™-TSA™ElectrocompetentCells(>2×1010cfu/µgpKanRDNA)areforusewithLucigen’spJAZZ®-OCorOKLinearVectorsintheBigEasyv2.0LinearCloningSystem.TheTSACellshavethetelNgenerequiredforhighefficiencytransformation,andtheantAgenerequiredforcopynumbercontrolwiththepJAZZvector,integratedintothehostgenome.ThesecellsalsogivehighyieldandhighqualityplasmidDNAduetotheendA1andrecA1mutations.Theycontainthemcrandmrrmutations,allowingmethylatedgenomicDNAthathasbeenisolateddirectlyfrommammalianorplantcellstobeclonedwithoutdeletionsorrearrangements.
Table1.E.cloniCompetentCellsforBAC,LargeorDifficultFragmentCloning
E.cloniCellLines | TransformationEfficiency | CloningMethylatedDNA | BAC,CosmidCloning | Blue/WhiteScreening |
BigEasy-TSAElectrocompetent | >2×1010 | YES | No | YES |
BigEasy-TSA:F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74endA1recA1araD139Δ(ara,leu)7697galUgalKrpsLnupG&lamBDa;-tonAAmpRsopABtelNantA