- Bestvalueforroutineproteinexpression...availableasElectrocompetentandChemicallyCompetentCells.
Lucigen’sE.cloniEXPRESSBL21(DE3)ElectrocompetentCellsarethefirsttoofferhighefficiencycloningandhighlevelproteinexpressioninthesamecell.Cloningefficienciesareincreased25-1,000foldrelativetocompetitors’preparationsofBL21cells(Figure1),whichisessentialforconstructionofcomplexexpressionlibraries.TheunprecedentedtransformationefficiencyofE.cloniEXPRESSElectrocompetentCells(>5×109cfu/µg)eliminatestheneedforplasmidtransferfromthecloningstraintotheexpressionstrain,savingdaysofworkinatypicalcloningandexpressionexperiment(Figure2).
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Figure1.TransformationefficiencycomparisonofcommerciallyavailableBL21(DE3)competentcells.
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Figure2.Comparisonofcloningandproteinexpressionprocedures. |
E.cloniEXPRESSBL21(DE3)strainscontainthephageT7RNApolymerasegenelinkedtotheIPTG-inducIBLepromoter,forusewithanyexpressionplasmidcontainingtheT7promoter.
NOTE:Forbestresultsinexpressingproteinsthatmaybetoxic,werecommendusingOverExpressCells.E.cloniEXPRESScellsarealsoavailableaschemicallycompetentcells.Withatransformationefficiencyof>1×107cfu/µgandveryeconomicalprices,EXPRESSChemicallyCompetentCellsoffersuperiorvalueforeverydayproteinexpressionwork.
ORDERINFORMATION
EachE.cloniEXPRESSKitcontains:theindicatedE.cloniEXPRESSElectrocompetentorChemicallyCompetentCellsinDUOpackaging(2transformationspertube),ExpressionRecoveryMedium(lactoseminus),pUC19PositiveControlPlasmid,andcompleteprotocols.
