Lucigen/Rec J核酸外切酶/RJ411250/250单位
市场价:
¥5560.00
美元价:
3336.00
产品分类:
DNA聚合酶
公司分类:
DNA_polymerase
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Magnesium-dependentdigestionofsingle-strandedDNAina5´to3´direction
- Specific:DigestsssDNAinthe5'to3direction
- HeatInactivated:Heatat65°Cfor20minutestokillenzymeactivity
- FlexIBLe:UsetoremoveprimersfromcompletedPCRortodegradesingle-strandedlinearDNAindsDNAandplasmidpreps
RecJExonuclease,derivedfromE.coli,catalyzesdegradationofssDNAina5´to3´direction.ItsactivityisdependentonMg2+.RecJExonucleasecanbeheat-inactivatedbyincubationat65°Cfor20minutes.
Applications
- RemovalofprimersfromcompletedPCR.
- Degradationofsingle-strandedlinearDNAindsDNAandplasmidpreps.
UnitDefinition:OneunitofRecJExonucleasecatalyzesthereleaseof1nmolofacid-solublenucleotidesfromactivatedsingle-strandedcalfthymusDNAin30minutesat37°Cunderstandardassayconditions.
StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),0.1mMEDTA,1mMDTT,0.1MNaCl,and0.1%Triton®X-100.
10XReactionBuffer:330mMTris-acetate(pH7.5),660mMpotassiumacetate,100mMmagnesium,acetate,and5.0mMDTT.
QualityControl:RecJExonucleaseisfreeofdetectableRNaseandcontaminatingDNAendonucleaseactivities.
ORDERINFORMATION
Includes10XReactionBuffer.品牌介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。