Lucigen/CircLigase™ II ssDNA Ligase/CL9025K/5,000 Units-免疫在线蚂蚁淘旗下平台

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商品详细Lucigen/CircLigase™ II ssDNA Ligase/CL9025K/5,000 Units

Lucigen/CircLigase™ II ssDNA Ligase/CL9025K/5,000 Units

商品编号： CL9025K

品牌： LGC

市场价： ¥16640.00

美元价： 9984.00

产地：美国(厂家直采)

公司：

产品分类： DNA修饰酶

公司分类： DNA_modification_enzyme

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

ATP-independent,non-catalyticThermostableligasethatcatalyzestheintramolecularligation(i.e.circularization)ofssDNAtemplates

Intramolecularligationofsingle-strandedDNAendscontaining5'-phosphatesand3'-hydroxylgroups
ATP-independent,non-catalyticenzyme
CircularizesssDNA>15baseslong
CompatIBLewithhightemperatureligationreactionsduetothermostABIlityofCircLigase™IIenzyme

Applications

ProductionofssDNAtemplatesforrolling-circlereplicationorrolling-circletranscriptionexperiments.
ProductionofssDNAtemplatesforRNApolymeraseandRNApolymeraseinhibitorassays.

CircLigase™IIssDNALigase*isathermostableenzymethatcatalyzesintramolecularligation(i.e.circularization)ofssDNAtemplateshavinga5´-phosphateanda3´-hydroxylgroup.IncontrasttoT4DNALigaseandAmpligase^®DNALigase,whichligateDNAendsthatareannealedadjacenttoeachotheronacomplementaryDNAsequence,CircLigaseIIssDNALigaseligatesendsofssDNAintheabsenceofacomplementarysequence.TheenzymeisthereforeusefulformakingcircularssDNAmoleculesfromlinearssDNA.CircularssDNAmoleculescanbeusedassubstratesforrolling-circlereplicationorrolling-circletranscription.

LinearssDNAof>15bases,includingCDNA,iscircularizedbyCircLigaseIIenzyme.Understandardreactionconditions,virtuallynolinearconcatamersorcircularconcatamersareproduced.InadditiontoitsactivityonssDNA,CircLigaseIIenzymealsohasactivityinligatingasingle-strandednucleicacidhavinga3´-hydroxylribonucleotideanda5´-phosphorylatedribonucleotideordeoxyribonucleotide.

CircLigaseIIhasgreateractivityandanewReactionBufferforimprovedligationefficiency.

UnitDefinition:OneunitofCircLigaseIIenzymeconverts1pmolofalinear5´-phosphorylatedCircLigaseIIStandard55-merOligointoanexonucleaseI-resistantcircularformin1hourat60°Cunderstandardassayconditions.

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,0.1mMEDTA,1mMDTT,and0.1%Triton^®X-100.

10XReactionBuffer:0.33MTris-Acetate(pH7.5),0.66Mpotassiumacetateand5mmDTT.

QualityControl:CircLigaseIIssDNALigaseisfreeofdetectablephosphatase,DNAexo-andendonuclease,andRNaseactivities.

ProductCitations

Polidoros,A.N.etal.(2006)BioTechniques41,35.
Shroff,H.etal.(2005)NanoLetters5(7),1509.
Lin,C.etal.(2006)AngewandteChemie118,7699.
Korlach,J.etal.(2008)Proc.Natl.Acad.Sci.USA105,1176.
McArthur,M.andBibb,M.J.(2008)Proc.Natl.Acad.Sci.USA105,1020.
Shroff,H.etal.(2008)BiophysicalJournal94,2179.
Kuhn,H.andFrank-Kamenetskii,M.D.(2008)NucleicAcidsRes.36,e40.
Murakami,T.et.al.(2008)NucleicAcidsRes.Doi:10.1093/nar/gkn1014

Figure1.CircLigase™IIssDNALigaseconvertslinearssDNA

ORDERINFORMATION

CircLigase™IIssDNALigaseisprovidedat100U/μl.

Contents:CircLigase™IIssDNALigase,CircLigase™II10XReactionBuffer,50mMMnCl₂,CircLigase™ssDNAControlOligo,Betaine,SterileWater.

品牌介绍

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。

公司介绍

品牌分类

灯泡和等温放大天然气产品缓冲液和试剂哺乳动物的表达 DNA核酸酶高效体外转录试剂盒大肠杆菌聚合酶 RNA扩增与标记

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