荧光素/核糖核酸酶I，E。大肠杆菌/N6901K/1000单位
市场价：
¥2060.00
美元价：
1236.00
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
RemoveunwantedRNAbydigestingwiththisheatlABIle,non-sequencespecificribonuclease
Complete:UnlikeRNaseA,RNaseIdoesnothaveabasepreferenceanddigestsbetweenalldinucleotidepairsinsingle-strandedRNA- HeatLabile:RNaseIiseasilyinactivatedbyheating70°Cfor15minutes
RNaseIdegradessingle-strandedRNAtonucleoside3´monophosphatesvia2´,3´cyclicmonophosphateintermediatesbycleavingbetweenalldinucleotidepairs,2,3unlikeRNaseA,whichcleavesonlyaftercytosineanduridine.Inaddition,theenzymeiscompletelyinactivatedbyheatingat70°Cfor15minutes,eliminatingtherequirementtoremovetheenzymepriortomanysubsequentprocedures.
Applications
- RemovalofRNAfromDNApreparations.1
- RNaseprotectionassaystodetectsingle-basepairmismatchesinRNA:RNAandRNA:DNAhybrids.1
UnitDefinition:OneunitofRNaseIdegrades100ngofE.coliribosomalRNApersecondintoacid-solublenucleotidesat37°Cunderstandardassayconditions.
DilutionandStorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,and0.1mMEDTA.
QualityControl:RNaseIisfreeofdetectableexo-andendodeoxyribonucleaseactivities.
References
- Johnson,M.(1996)EpicentreForum3(4),7.
- Shen,V.andSchlessinger,D.(1982)TheEnzymesXV(PartB),501.
- delCardayré,S.B.andRaines,R.T.(1995)Anal.Biochem.225,176.
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| Figure1.RemovalofRNAfromplasmidDNApreparations.PlasmidDNAwaspreparedfrom1.5mLofanovernightculture,sUSPendedin50µLTEbuffer(10mMTris-HCl(pH8),1mMEDTA),andtreatedwithRNaseA,RNaseI,ornoenzyme.FiveµLofeachreactionwereresolvedbyagarosegelelectrophoresisandvisualizedbystainingwithethidiumbromide.Lane1,noRNase;Lane2,1.5UofRNaseI;Lane3,20µg/mLRNaseA;MW,1-kbladder.ArrowdenotessmallundigestedRNA. |
ORDERINFORMATION
Providedwithdilutionbuffer,10XTNEbuffer,and0.1MDTT.品牌介绍
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
