- SynthesizeRNAwithqualityNTPsolutions
- StABIlizeRNAbyincorporating2’-fluorineduringinvitrotranscription
NTPand2’-Fluorine-Nucleoside-5’-TriphosphatesolutionsareprovidedasRNase-andDNase-free,neutralsolutionsthatmeetstrictspectrophotometriccriteriaandaretestedinfunctionalassays.
NTPsolutionsidealforuseinRNAsynthesisreactions. ThesenucleotidesareprovidedasfourseparatetubesofATP,CTP,GTP,andUTPat100mMconcentration(25µmol/tube).
TheATPSolutionisavailableseparatelyandissuitableforuseasacofactorwithT4DNALigaseforligatingDNAmoleculesandT4PolynucleotideKinaseforphosphorylatingthe5′endsofDNAorRNAmolecules,andforRNAtailingapplications. ATPSolutionisprovidedasa100mMsolution(25µmol/tube).
2’-Fluorine-dCTPand2’-Fluorine-dUTPareefficientlyincorporatedintoRNAtranscriptsbyinvitrotranscriptionreactionswithDurascribe®T7RNAPolymerase. Completesubstitutionofthe2′-Fluorine-dCTPand2′-Fluorine-dUTPforthecanonicalCTPandUTPinaDurascribe®invitrotranscriptionreactionyields“DurascriptRNA”thatiscompletelyresistanttodigestionbyRNaseA.DurascriptRNAissuitableforRNAiandRNAaptamerapplications. Thesenucleotidesaresuppliedasneutralizedsolutionsat50mMeach(1µmol/tube).
ATP(Adenosine-5´-Triphosphate)
| CTP(Cytidine-5´-Triphosphate) | GTP(Guanosine-5´-Triphosphate)
|
UTP(Uridine-5´-Triphosphate) | 2´-Fluorine-dUTP(2´-F-dUTP)
| 2´-Fluorine-dCTP(2´-F-dCTP)
|
ORDERINFORMATION
2’-Fluorinenucleotidesolutionsaremadewithsteriledeionizedwaterat50mMeachandadjustedtopH7.0withNaOH.
NTPandATPSolutionsareprovidedinsteriledeionizedwaterat100mMandadjustedtopH7.0withNaOH.
