Lucigen/快速提取物™ RNA提取试剂盒/QER090150/50 ml（500次提取）
市场价：
¥10560.00
美元价：
6336.00
产品分类：
其他试剂
公司分类：
Other_reagents
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
RapidextractionofRT-PCR-readyRNAfromculturedmammaliancells
Fast:Extractend-pointandreal-timeRT-PCRreadyRNAinminutesfromlowtohighnumbersofsamples- Simple:Singletubeprotocol
- CompatIBLe:ExtractedRNAiscompatiblewithbothreal-timeandendpointRT-PCR
- Automation-friendly:Simpleprotocolmakesincorporationintoanautomatedworkfloweasy
- Flexible:ADnaseIdigestionstepcanbeaddedforsensitivedownstreamapplicationswhennecessary
- Safe:Notoxicreagentsused
TheQuickExtract™RNAExtractionKitisafast,simplewayofpreparingRNAforRT-PCR(bothend-pointandreal-time).Thesingle-tubesystemrequiresonlyvortexmixingtolysethecells,andpreparetheRNAforCDNAsynthesis.Theresultiseasyprocessingofonetohundredsofsamplesinminutes,withnosamplelossortoxicorganicsolvents.TheQuickExtractRNAExtractionSolutionworkswithculturedadherentandsUSPensioncellsincludingbuccalcells,andhasbeentestedonhuman,mouse,rat,E.coli,andS.aureuscellcultures.Itisnotsuitablefortissuesamplesorplantsamples.AnoptionalDNaseItreatmentmayimprovecertaindownstreamapplications.
Applications
- PreparationofRNAfromculturedadherentandsuspensioncellsforRT-PCR.
 | Figure1.End-pointRT-PCRofdifferentregionsofa14kbmessageusingHeLacellextractwiththeQuickExtract™RNAExtractionKit.Asamplecontaining105HeLacellswaslysedin100µLofQuickExtractRNAExtractionSolutionbyvortexmixing.ThelysatewasreversetranscribedwiththeMMLVReverseTranscriptase1stStrandcDNASynthesisKitusingstandardconditionsandrandomprimers.ThecDNAwasthenamplifiedwithsixprimersetstop532usingtheFailSafe™PCRSystem.LaneM,100 bpladder;Lane1,12984to13892;lane2,9406to10202;lane3,5194to5802;lane4,4191to4690;lane5,2280to2676;andlane6,1029to1329. |
 | Figure2.ComparativeyieldofRT-PCRproductwithdifferentRNAextractionkits.Lysateswerepreparedaccordingtomanufacturers'instructionsandusedastemplatetoproducecDNAusingtheMMLVRT1stStrandcDNASynthesisKit,followedbyPCRusingtheFailSafe™PCRSystemwithprimersfortheALDOAgene.Productswereseparatedona2%agarosegelandstainedwithSYBR®gold.LaneM,100bpladder;lanes1and2,QuickExtract™RNAExtractionKit;lanes3and4,kitfromVendor1;lanes5and6,kitfromVendor2. |
品牌介绍
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
