卢西根/马斯特纯™ 酵母DNA纯化试剂盒/MPY80200/200纯化
市场价：
¥7420.00
美元价：
4452.00
产品分类：
DNA提取纯化试剂
公司分类：
DNA_extraction
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
ObtainhighyieldsofqualityDNAfromyeastwiththiseasytousekit
Fast:ObtainpurifiedDNAinlessthan40minutes- FlexIBLe:PurifyDNAfromawidevarietyofyeastspecies,includingCandida,Saccharomyces,Pichia,andSchizosaccharomyces,andfilamentousfungisuchasAspergillus2andPenicillium
- HighQualityDNA:PurifyhighmolecularweightgenomicDNAthatisreadytouseinmanymolecularBIOLOGyapplications,includingPCRamplification,restrictionendonucleasedigestion,Southernblotting,genomiclibrarypreparationandfungalidentificationandtyping
- HighYields:PurifymoreyeastgenomicDNAthanothercommerciallyavailablekits
- Simple:Noenzymaticlysis,beadbeating,columns,phenolorotherorganicsolventsareused
TheMasterPure™YeastDNAPurificationKitenablesefficient,high-yieldpurificationofhigh-molecular-weightDNAfromyeastandotherfungi.Theprotocolinvolvesnonenzymaticcelllysisat65°C,followedbyremovalofproteinbyprecipitation,andnucleicacidprecipitationandresUSPension.Nolyticase,proteolyticenzymes,orbead-beatingareusedintheprocedure.YeastgenomicDNAyieldsusingtheMasterPureKitaremuchhigherthanyieldsobtainedwithothercommerciallyavailablekits(Fig.1).1Theprotocolcanbeeasilyadjustedforlargerorsmallersamples,includingsingleyeastcolonies.1Therecoverednucleicacidcanbeuseddirectlyinmostapplications,includingPCRamplification.
References
- Hoffman,L.andMoan,E.(1998)EpicentreForum5(4),1.
- Jin,J.,Lee,Y.-K.andWickes,B.L.(2004)J.ClinMicrobiol.42,4293.
| Figure1.ComparativeyieldsofDNAfromtheMasterPure™YeastDNAPurificationKitandcompetitorkits.DNAwasquantitatedspecificallywithHoechstfluorescentdye33258,whichgivesminimalfluorescencewithRNA.ThedatarepresenttheaverageDNAyieldsdeterminedbyfluorometryfromtwoexperimentswithS.cerevisiaeandC.albicans.TheMasterPureKitproducedupto17timesmoreDNAfromC.albicansand12timesmoreDNAfromS.cerevisiaethanotherkits. |
 | Figure2.SizedistributionofDNAproducedfromtheMasterPure™Kit.Foreachkitindicated,a500-ngaliquotofpurifiedyeastDNAwasanalyzedbypulse-fieldgelelectrophoresisona1%agarosegel.Thegelwasstainedwithethidiumbromide.ThemajorityofDNAisolatedwiththeMasterPureKitwasestimatedtobeinthe40-to50-kbsizerange,whiledegradationtosmallerfragmentswasobservedwithotherkits.LaneM,lamBDaDNAladder;Lane4,T7DNA(40kb). |
ORDERINFORMATION
Contents:YeastCellLysisSolution,MPCProteinPrecipitationReagent,TEBuffer,RNaseA.品牌介绍
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
