- OutstandingreliABIlityandreproducibilityinColonyPCR
- IdealforPCRgenotyping
- Convenient1XMasterMix:justaddacolony,primers,andcycle.
- HighThermostabilityandoptimizedformulationfor98Cdenaturationtohandlethetoughesttargets.
- ContainsGreentrackingdyefordirectgelloADIng
FreeSampleAvailable
Finally,aPCRenzymespecificallyformulatedforColonyPCR.
Reliableperformancefromcolonies.Bypassallcelllysisandsampleprepwithoutworryingaboutfailedreactions.Outstandingreproducibilitytogetyourampliconthefirsttime.(Figure1).
Extremethermostability. Cycleat98°Ctolysecellsanddenaturethetoughesttemplates.TheaddedPCRenhancerimprovesamplificationandthermostabilitytogiveyouthebestPCRproductpossIBLe.
Toughtemplatesarenomatch: GC-richDNAcanmakecolonyPCRthatmuchmore difficult–andCloneIDcanhandleitwithease.Cleanandrobustamplificationontargetswhereotherenzymesfallshort(Figure2)
Theeasiestworkflow. CloneIDisnotonlya1Xmastermixthatonlyrequiresacolonyswipeandprimers,butitalsocontainsaGreentrackingdyesoyoucandirectlyloadyourPCRproductontoagel.
 |
| Figure1.CloneID™1XColonyPCRMasterMixwasusedon96uniquecoloniesfromapetridishcontainingalibrarysample.Successratewas97%(93outof96)foramplification. |
.
 |
| Figure2.CloneID™1XColonyPCRMasterMixiscomparedwithotherPCRproducts(CloneID™,Platinum®PCRSupermix,OneTaq®,KAPA2G)claimingstrongColonyPCRperformance.The12targetsaregenesfromCellulomonasfimi,aspecieswhosegenomeis75%GC,whichwereclonedintoastandardplasmid. |
ORDERINFORMATION
CloneID™1XColonyPCRMasterMixisprovidedina1XMasterMixformatthatwithstands98°Cdenaturation.Allrequiredbuffercomponents,enzyme,Mg++anddNTP’sareallpresentinoptimizedconcentrations.TheproductalsocontainsGreentrackingdyeforconvenientgelloading.Onlyasmallamountofwholecellsfromacolonyandprimersarerequiredtosetupthereaction.Pleaseseetheusermanualforcompositioninformation.Standardreactionsizeis25µL.
ThisproductisprimarilyintendedforE.colicoloniesorpurifiedDNAtemplates.CloneIDhasnotbeentestedfordiverseorganisms.
