- OutstandingperformanceandvalueareperfectforroutinePCR.
- Canbecycledupto98°CtoamplifythemostchallengingGC-richtemplatesotherscannot.
- Ready-to-usePCRreactionmastermixes,containdNTPsandPCREnhancer.
- Non-proofreADIngpolymerase
- EconoTaqPLUSGREENcontainstrackingdyesforgelelectrophoresis
- MoreeffectivethanotherTaqmastermixes.EconoTaqPLUSandPLUSGREEN2XMasterMixescontainaproprietaryPCRenhancer,sotheycansuccessfullyamplifytemplatesthatfailtoamplifywithotherPCRmastermixes(Figure1).HighdensityreactionbufferenablesdirectgelloadingfollowingPCR.
- ExcellentresultsinroutinePCR.EconoTaqPLUSGREEN&EconoTaqPLUScontainhighpurity,highactivityEconoTaqDNAPolymeraseforreliableamplificationoftemplatesupto5kb(Figure1).
- AmplifychallengingGC-richtemplates.These2XMasterMixescanbecycledupto98°CtoamplifychallengingGC-richtemplatesotherscannot(Figure2).
- ConvenienttrackingdyesinEconoTaqPLUSGREEN.Ona1%agarosegel,thebluedyemigratesatthesamerateasa5kbDNAfragment,andtheyellowdyemigratesat75bp.SeeFigure3.Ifdesired,thedyesareeasilyremovedbystandardDNApurificationproductsorethanolprecipitation.Forfluorescenceorabsorbancedetectionassays,EconoTaqPLUS2XMasterMixoffersthesamegreatperformancewithoutthedyes.
- FlexIBLe&easytouse.EconoTaqPLUSGREENandEconoTaqPLUSarepackagedin50reactionvialsthatcanbestoredintherefrigerator(+4ºC)forupto3months.Theyarestableforatleast10freeze-thawcycles.
- Lowprice!EconoTaqPLUSGREENisonly26¢per50µlPCRreaction…foreverything!EconoTaqPLUSGREEN&EconoTaqPLUSoffergreatvaluewhencomparedtoTaqmastermixes. EconoTaqPLUSGREEN&EconoTaqPLUSprices(see“OrderingandPricing”)arebasedona50µlPCR;usinga25µlreactionvolumewillstretchyourresearchbudgetevenfurther.
FreeSampleAvailable
EconoTaq®PLUSGREEN&EconoTaqPLUS2XMasterMixesofferPerformance,Convenience,ReliABIlity,andValueforRoutinePCR.
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Figure1.EconoTaqPLUSGREENMasterMixvs.GoTaq®Greenmastermix(Promega)inPCR.PCRwasperformedaccordingtothemanufacturer’srecommendationsusingprimersspecificfortheindicatedgenes.PanelA,0.7kbprokaryoticsingle-strandedDNAbindingprotein(genomicDNA);PanelB,2.7kbprokaryoticDNApolymeraseA(genomicDNA);PanelC,4.5kbgene(plasmidDNA). |
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Figure2.GC-richregionsfromhumangenomicDNAwerePCRamplifiedusing98°Cdenaturation. |
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Figure3.EasyvisualizationwithEconoTaqPLUSGREEN.Duringelectrophoresis,thegreenloadingcolorseparatesintoaslow-movingbluedyeandafast-movingyellowdye. |
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PleaseNote:
SomeapplicationsinwhichLucigen'sEconoTaqPLUSGREENandEconoTaqPLUS2XMasterMixescanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandcertainothercountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainapatentlicensedependingupontheparticularapplicationinwhichtheproductisused.ThePCRprocessisthesubjectofEuropeanPatentNos.201,184and200,262ownedbyHoffman-LaRoche.ThosepatentsexpiredonMarch28,2006.ThecorrespondingPCRprocesspatentsintheUnitedStatesexpiredonMarch29,2005.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.
ORDERINFORMATION
UseEconoTaqPLUSGREEN2XMasterMixwhenthePCRproductswillbeanalyzedbyagarosegelelectrophoresisandethidiumbromidestaining.UseEconoTaqPLUS2XMasterMixwhenthePCRproductswillbeanalyzedbyabsorbanceorfluorescenceexcitation,toavoidpossibleinterferencebythedyes.EconoTaqPLUSGREEN&EconoTaqPLUSpricesarebasedona50µlPCRreaction.
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