MasterAmp™TaqDNAPolymeraseisaThermostableDNApolymerasederivedfromThermusaquaticus.Havingoptimalactivityattemperaturesabove70°C,itwasthefirstthermostableenzymeusedforPCR.Ithasanintrinsic5´to;3´structure-dependentexonucleaseactivity,butlacks3´to;5´proofreADIngexonucleaseactivity.TheenzymeishighlypurifiedusingaproprietaryprocedurethatvirtuallyeliminatescontaminatingbacterialDNA.

Applications

• PCRandmultiplexPCRamplificationofDNAtemplates.

UnitDefinition:OneunitofMasterAmpTaqDNAPolymeraseconverts10nmolofdNTPsintoacid-insolublematerialin30minutesat70°Cunderstandardassayconditions.

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,0.1mMEDTA,0.5%Tween20,0.5%NP-40,and1mMDTT.

QualityControl:MasterAmpTaqDNAPolymeraseistestedfortheabsenceofdetectablenonspecificexo-andendonucleaseandRNaseactivities,andarefunctionallytestedinPCR.

*PurchaseofMasterAmp™TaqDNAPolymeraseincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser'sowninternalresearch.Nootherpatentrights(suchas5´NucleaseProcesspatentrights)areconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.Coveredbyissuedandpendingpatents.Forcompletelicensestatement,seep.iv.

**Coveredbyissuedand/orpendingpatents.