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Lucigen/MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit/MBCL90310/10 Reactions
Lucigen/MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit/MBCL90310/10 Reactions
市场价：
¥4520.00
美元价：
2712.00
产品分类：
文库
公司分类：
Gene_library
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
ProduceamplifiedamountsofCDNAfromprecious,limitingcellsampleswithoutintroducingbias.
- PerformRNAamplificationdirectlyfromculturedcelllysatesfollowedbycDNAsynthesis
- Amplifywiththishigh-fidelity,linearRNAamplificationprocessthatpreservestherelativetranscriptabundanceofthesample.
- Getmoredataoutofprecioussamples–uselessRNAformoreRT-qPCRreactions
- ReADIlyandreproducIBLydetectevenlow-abundancetranscriptsfromaslittleasonecell.
- NoneedtopurifyRNA:PerformRNAamplificationandcDNAsynthesisreactionsdirectlyfromcelllysates.
- CollectsampleslessoftenandarchivecDNAforfutureuse,savingtimeandeffort.
Applications
- AmplificationofmRNAdirectlyfromacelllysate,withouttheneedforpurifyingRNA,andconversionoftheamplifiedRNAtocDNA.
- SignificantlyincreasingthenumberandsensitivityofqRT-PCRsfromverysmallnumbersofcells.
- GenerationoflargeamountsofcDNAfromthemRNAofverysmallsamplesforarchivalpurposes.
TheMessageBOOSTER™cDNASynthesisfromCellLysatesKitenablestheusertoperformsensitiveqRT-PCRstudiesfromverysmallpopulationsofcells,evenfromaslittleasonecell. ThekitamplifiesthecellularmRNAdirectlyfromacelllysate,withouttheneedtopurifytotalRNA,andthenconvertstheamplifiedRNAtocDNAthatisreadyforqPCR(Fig.1).AmplifiedcDNAisefficientlyproducedfromthelysatesof1to500cells. |  | Figure1(clicktoenlarge).AnoverviewoftheprocedurefortheMessageBOOSTER™cDNASynthesisfromCellLysatesKit.Akitreactionamplifiesthepoly(A)RNA(mRNA)directlyfromacrudecelllysatewithouttheneedforRNApurification.TheamplifiedRNAisthenreverse-transcribedtocDNAthatcanbedilutedupto1,000-foldforqPCR. |
 | Figure2.AMessageBOOSTER™KitreactionproducessufficientcDNAfromasingle-celllysateforthousandsofsensitiveqPCRs.qPCRwasperformedusingundiluted(red),1:10diluted(green),1:100diluted(blue),and1:1,000diluted(purple)cDNAfromalysateofasingleNRKcell.Thelow-abundancePBGDtranscriptwasreadilydetected. |
ORDERINFORMATION
Contents:QuickExtract™RNAExtractionSolution,MessageBOOSTER™T7-Oligo(dT)Primer,MessageBOOSTER™ReverseTranscriptionPreMix,RNaseInhibitor,MMLVReverseTranscriptase,MessageBOOSTER™DNAPolymerasePreMix,MessageBOOSTER™DNAPolymerase,MessageBOOSTER™cDNAFinishingSolution,MessageBOOSTER™InVitroTranscriptionPreMix,MessageBOOSTER™T7RNAPolymerase,MessageBOOSTER™T7TranscriptionBuffer,RNase-FreeDNaseI,RandomPrimers,RNaseH,ForwardControlPCRPrimer,ReverseControlPCRPrimer,100mMDTT,RNase-FreeWater,Poly(I).品牌介绍
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
