Lucigen/MessageBOOSTER&trade；qPCR/MB060124/24反应cDNA合成试剂盒
市场价：
¥10520.00
美元价：
6312.00
产品分类：
文库
公司分类：
Gene_library
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
ProduceamplifiedamountsofCDNAfromprecious,limitingtotalRNAsampleswithoutintroducingbias.
- PerformRNAamplificationonpurifiedtotalRNAfollowedbycDNAsynthesisanddetection
- Amplifywiththishigh-fidelity,linearRNAamplificationprocessthatpreservestherelativetranscriptabundanceofthesample.
- Getmoredataoutofprecioussamples–uselessRNAformoreRT-qPCRreactions
- ReADIlyandreproducIBLydetectevenlow-abundancetranscriptsinRNAfromasinglecell(CTvalues<35cycles)
- CollectsmallRNAsampleslessoften.
- FastprotocolamplifiesandsynthesizescDNAinonly1day.
Applications
TheMessageBOOSTER™cDNASynthesisKitforqPCRenablestheusertoperformsensitiveqPCRamplificationsusingthetotalRNAfromverysmallpopulationsofcells,evenfromaslittleasonecell(Table1). ThekitamplifiesthemRNAcontainedinasmalltotalRNAsampleandthenconvertstheamplifiedRNAtocDNAthatisreadyforPCRorqPCR(Fig.1).TheMessageBOOSTERcDNASynthesisKitforqPCRusesoligo(dT)toprimecDNAsynthesis.Therefore,thiskitisbestusedwithintacttotalRNApreparations. |  | Figure1(clicktoenlarge).OverviewoftheMessageBOOSTER™cDNASynthesisKitprocedure.ThekitusesalinearRNAamplificationprocesstoamplifytheRNAfromasmallpopulationofcells,thenconvertstheamplifiedRNAtocDNAthatisreadyforPCR.Theprocedurecanbecompletedin1day. |
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| Figure2.SensitivityoftheMessageBOOSTER™cDNASynthesisKit.cDNAproducedfrom10pgoftotalNRKRNAsignificantlyimprovedthesensitivity(loweredtheCT)ofdetectingthelow-abundancePBGDtranscriptcomparedtocDNAproducedfrom10pgofunamplifiedRNA. | ||
| Table1.ThenumberofqPCRamplificationsthatcanbeperformedusingcDNAproducedbyaMessageBOOSTER™cDNASynthesisKit.ThenumberofqPCRsisdependentontheamountofinputtotalRNAandtheabundanceofthetranscript(s)ofinterest. | ||||||||||||||
a.Low-AbundanceTranscripts=1-1,000copiespercell | |||||||||||||||
ProductCitations
- Grunenwald,H.etal.(2006)EpicentreForum13(3),22.
- DeFazio,R.A.etal.(2006)J.Neuroscience26,3971.
- Kaeffer,B.etal.(2007)PediatricResearch62,564.
- Lochner,M.,etal.(2008)J.Exp.Med.205,1381.
- Graham,D.M.etal.(2008)J.Neurophysiology,99,2522.
- Liu,X.et.al.(2009)Am.J.Physiol.LungCellMolPhysiol.296,L158
- Yakovlev,I.A.etal.(2008)FungalGeneticandBIOLOGy45,498
- Jung,Y.etal.(2008)StemCellsExpress10,1634
- Xi,D.etal.(2008)J.NeurosciMethod10,1016
- Michel,M-L.etal.(2008)Proc.Nat'lAcadSci-USA105(50),19845
- Satoh-Takayama,N.etal.(2008)Immunity29,958
- Bouskra,D.etal.(2008)Nature10,1038
- Yakovlev,I.A.etal.(2008)FungalGeneticsandBiology45,498
- Roberts,C.D.et.al.(2009)J.Physiology587,1657
- Peduto,L.et.al.(2009)J.Immunology182,5789
- Xi,D.et.al.(2009)Int'lJ.Neuropsychopharmacology,May13:1-14
ORDERINFORMATION
MessageBOOSTER™T7-Oligo(dT)PrimerA,RNaseInhibitor,MessageBOOSTER™ReverseTranscriptionPreMix,MessageBOOSTER™DNAPolymerasePreMix1,MessageBOOSTER™DNAPolymerase1,MessageBOOSTER™cDNAFinishingSolution,MessageBOOSTER™RandomPrimers,MessageBOOSTER™RNaseH,MMLVReverseTranscriptase,MessageBOOSTER™InVitroTranscriptionPreMixA,MessageBOOSTER™T7RNAPolymerase,MessageBOOSTER™T7TranscriptionBuffer,RNase-FreeDNaseI,ForwardandReverseControlPCRPrimers,DTT,RNase-FreeWater,Poly(I),NRKTotalRNAControl.品牌介绍
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
