Lucigen/EZ-Tn5&trade&书信电报；KAN-2>；Tnp Transposome&trade；试剂盒/TSM99K2/10-免疫在线蚂蚁淘旗下平台

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商品详细Lucigen/EZ-Tn5&trade&书信电报；KAN-2>；Tnp Transposome&trade；试剂盒/TSM99K2/10

Lucigen/EZ-Tn5&trade&书信电报；KAN-2>；Tnp Transposome&trade；试剂盒/TSM99K2/10

商品编号： TSM99K2

品牌： LGC

市场价： ¥10000.00

美元价： 6000.00

产地：美国(厂家直采)

公司：

产品分类：转染试剂

公司分类： Transfection

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Generaterandomgeneknockoutsinlivingbacteria

Generatemutantswithimprovedgeneticsorfunctionacrossabroadhostrangeofbacterialcelltypes
Characterizenovelgenesandgenefunctions
Identifygenesinvolvedinpathogenesis,toxicity,biofilmdevelopment
Unravelmetabolicpathways
Identifyessentialgenesandregulatoryelements
100’sofcitationsformanydifferentapplications

Applications

Rapidgenerationofknock-outmutantsinbacterialcells.
Knock-inofgenesforbacterialstraindevelopment.
"Tagging"bacteriawithvisIBLegeneticMarkersforenvironmentallocalizationstudies.
DirectsequencingofbacterialchromosomalDNA.

EZ-Tn5™Transposome™complexesareformedbetweenanEZ-Tn5™TransposonandEZ-Tn5™Transposase,andprovideasimpleandreliablemethodforgeneratingalibraryofrandomgeneknockoutsinvivo.*JustelectroporatetheEZ-Tn5TransposomeintoanyofabroadrangeoflivingbacterialcellsandselectforamarkerencodedbytheEZ-Tn5Transposon(Fig.1).Becausethereisnoneedforcellconjugation,suicidevectors,orspecifichostfactors,EZ-Tn5Transposomesareidealforcreatingmutantsinspeciesthathavepoorlydescribedgeneticsystemsorlackadequatemoleculartools.

Ready-to-useEZ-Tn5Transposomes*areavailablecontainingakanamycinselectablemarker(<KAN-2>).ThismarkerisreADIlyexpressedinmanyGram-negativebacteria.YoucanalsocreateyourownEZ-Tn5TransposomeusingoneoftheEZ-Tn5pMOD™TransposonConstructionVectorsandEZ-Tn5Transposase.

Figure1.TheEZ-Tn5™TransposoninsertionsiteinbacterialDNAcanbesequenceddirectlyusinggenomicDNAisolatedusingtheMasterPure™CompleteDNAPurificationKitandprimershomologoustotheendsofthetransposon.

AllEZ-Tn5Transposonscontainuniqueprimer-bindingsitesateachendforbidirectionalsequencing.Hence,ageneknockoutcanbesequenceddirectlyusingbacterialgenomicDNAastemplateandtheprimersprovidedwitheachTransposome.TransposoninsertionsmadeusinganEZ-Tn5<R6Kγori/KAN-2>TnpTransposomeKitcanberescuedandtheflankingDNAsequenced.

EZ-Tn5Transposome-mediatedinsertionshavebeenmadeinmanydifferentmicroorganisms,includingAcinetobactor,Campylobacter,Escherichia,Mycobacterium,Proteus,Pseudomonas,Saccharomyces,Salmonella,Trypanosoma,Xylella,andothers.Thenumberoftranspositionclonesobtainedishighlydependentonthetransformationefficiencyofthehostcell(Table1).

Benefits

Rapidmutagenesisprocedureissimplerandeasiertousethanchemicalmutagenesis.
Moreefficientthanusingmini-transposonswithsuicideplasmids.
Broadhostrange:over60speciesofGram-negativeandGram-positivebacteriatransposedsofar.

Table1.ExamplesofbacterialstrainsmutagenizedusingtheEZ-Tn5™Tranposomes™
Actinobacilluspleuropneumoniae Agrobacteriumtumefaciens Bacillussubtilis Bartonellahenselae BDellovibriobacteriovorus Campylobacterjejuni Clavibactermichiganensis subsp.sepedonicus CoryNEBacteriumdiphtheriae Enterobactercloacae Escherichiacoli Francisellatularensis Haemophilusducreyi Moraxellacatarrhalis Mycobacteriumavium Mycobacteriumbovis(BCG)	Mycobacteriumtuberculosis Myxobacteriumangiococcus Neisseriagonorrhoeae Pseudomonasputida Pseudomonassyringae Rhodococcusequi Rickettsiaprowazekii Salmonellatyphimurium Serratiamarcesens Silicibacterpomeroyi Spiroplasmacitri Streptococcuspyogenes Xanthomonascampestris Xylellafastidiosa Zymomonasmobilis

Table2.NumberofKan^Rtransposoninsertionclonesproducedfromelectroporationof1µlofEZ-Tn5™<KAN>TnpTransposome™
E.coli	>10⁵	Proteusvulgaris	>10³
Salmonellaty.	>10⁴	Mycobacteriumsmegmatis	>10²
Pseudomonassp.	>10²

*Coveredbyissuedand/orpendingpatents,exclusivelylicensedorassignedtoEpicentre®(anIllumina®Company).

ORDERINFORMATION

EZ-Tn5™TnpTransposome™,KAN-2FP-1ForwardPrimer,KAN-2RP-1ReversePrimer,andSterileWater.

品牌介绍

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。

公司介绍

品牌分类

灯泡和等温放大天然气产品缓冲液和试剂哺乳动物的表达 DNA核酸酶高效体外转录试剂盒大肠杆菌聚合酶 RNA扩增与标记

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