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主营:主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
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当前位置: 首页 > 产品中心 > Transfection > 卢西根/TransforMax™EC100D™pir-116电子兼容大肠杆菌/EC6P095H/5 x 100µl
商品详细卢西根/TransforMax™EC100D™pir-116电子兼容大肠杆菌/EC6P095H/5 x 100µl
卢西根/TransforMax™EC100D™pir-116电子兼容大肠杆菌/EC6P095H/5 x 100µl
卢西根/TransforMax™EC100D™pir-116电子兼容大肠杆菌/EC6P095H/5 x 100µl
商品编号: EC6P095H
品牌: LGC
市场价: ¥4520.00
美元价: 2712.00
产地: 美国(厂家直采)
公司:
产品分类: 转染试剂
公司分类: Transfection
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

RescuecloningofEZ-Tn5™transposedelements

  • RescuetranspositionmutantscreatedusingtheEZ-Tn5™R6KɣoriKan-2>InsertionKit
    orTnpTransposome™KitFormerly from Epicentre
  • PropagateplasmidstaggedwiththeEZ-Tn5™R6KɣoriKan-2>InsertionKit

TheTransforMax™EC100D™pir+ElectrocompetentE.coliandTransforMax™EC100D™pir-116ElectrocompetentE.colieachconstitutivelyexpresstheπprotein(thepirgeneproduct)forreplicationofplasmidscontainingtheR6Kγoriginofreplication(R6Kγori).ThecellsarederivedfromEpicentre'sTransforMax™EC100™ElectrocompetentE.colibyP1phagetransductionwithastraincontainingthepir+orpir-116genelinkedtoadihydrofolatereductase(DHFR)Marker.

TheTransforMaxEC100Dpir+cellswillmaintainR6Kγoricontainingplasmidsatapproximately15copiespercell,1forcloningofpotentiallytoxicorunstableDNAsequences.TheTransforMaxEC100Dpir-116cellsareforhigh-copypropagationofupto250rescueplasmidcopiespercell.1

Benefits

  • AcceptslargeclonesforunbiasedpropagationandstABIlityoflargerescueclones.
  • lacZΔM15forblue/whitescreeningofrecombinants.
  • Restrictionminus[mcrA,Δ(mrr-hsdRMS-mcrBC)]enablesefficientcloningofmethylatedDNA.
  • Endonucleaseminus(endA1)toensurehighyieldsofDNA.
  • Recombinationminus(recA1)forgreaterstabilityoflargeclonedinserts.

Genotypes

TransforMaxEC100Dpir+ElectrocompetentE.coli:
F-mcrAΔ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(StrR)nupGpir+(DHFR)

TransforMaxEC100Dpir-116ElectrocompetentE.coli:
F-mcrAΔ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(StrR)nupGpir-116(DHFR)

TransformationEfficiency
Greaterthan5×109colonyformingunits(cfu)/µgwithpR6KancontrolDNA.

References

  1. Metcalf,W.W.etal.(1994)Gene138,1.

ORDERINFORMATION

Eachproductcontains5tubeseachcontaining100µLofelectrocompetentcells,whichisenoughcellsfor10electroporations.AcontrolvectorcontainingandR6Kγoriisalsoincludedwitheachproduct.
品牌介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。