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Lucigen/End-It™ DNA End-Repair Kit/ER81050/50 Reactions
来自 : 发布时间:2024-04-29

Convert DNA with damaged or incompatible 5´- and/or 3´-protruding ends into ready-to-ligate, 5´-phosphorylated, blunt-ended DNA.

  • \"FormerlyComplete conversion of DNA into blunt-ended, phosphorylated DNA
  • Repair sheared, nebulized or restriction enzyme-digested genomic DNA
  • Prepare templates for NGS (next-generation sequencing) or shotgun library preparation

Applications

The End-It™ DNA End-Repair Kit converts DNA containing damaged or incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated, blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA Ligation Kit.

The End-It DNA End-Repair Kit contains reagents for 20 or 50 end-repair reactions (repair of up to 100 µg or 250 µg of genomic DNA, respectively).

\"\"
Figure 1. DNA fragments containing any type of ends are rapidly and efficiently converted to 5´-phosphorylated, blunt-ended DNA using the End-It™ DNA End-Repair Kit. The end-repaired DNA is ready for blunt-end ligation using, for example Epicentre\'s Fast-Link™ DNA Ligation Kit.

ORDER INFORMATION

Contents: End-Repair Enzyme Mix, End-Repair 10X Buffer, dNTP Solution, ATP.

本文链接: http://lucigen.immuno-online.com/view-1501856492.html

发布于 : 2024-04-29 阅读()